MSC Culture Supplement

Coping with Variations

Due to its self-renewal capacity, multilineage differentiation potential ,and immunomodulatory properties, mesenchymal stem cells have become candidates for cellular therapy of several diseases in humans.

However, while ISCT has defined MSC by the cell surface markers (CD105+, CD73+, CD90+, CD34-, CD14-, or CD11b-, CD79- or CD19-, HLA-DR-), properties of MSC still vary largely by the deriving tissue, the donor and the isolation technique applied (ref. 1). Since most MSCs progressively lose their multipotency and proliferation capacity in dishes, such inherent variation in cell properties has a great influence on the culture efficiency and quality of cultivated cells and hence the clinical outcome.

It is, therefore crucial to use standardized culturing technique and culture medium to yield consistent cultural results along with the project development from bench to bedside.


From Xeno-Free to Animal-Free

Today, it remains challenging to replace undefined components such as fetal bovine serum (FBS) or human platelet lysate (hPL) with a completed chemical-defined medium having comparable cell growth performance for MSC. While DOE methodologies are powerful in the optimization of the composition of components, the growth-promoting ability, i.e., the potency, of individual reagent is subjected to its manufacture.

Albumin is one frequently used component, and it has been shown critical to the growth of MSC (ref. 2). However, owing to its history as a by-product in the plasma fractionation process and its stickiness toward other plasma molecules, albumin has long been considered to have untenable lot-to-lot variations. The influence of albumin's impurities, such as fatty acids, on MSC culture, is also reported (ref. 3).


deAlbumin™ is the formulated recombinant rHSA derived from yeast, on which all small molecules associated are stripped and re-associated from a defined recipe of such small molecules. deAlbuminTM can boost the MSC cell by 30-47%.

Certified Raw Material

deAlbumin™ product, DA01, is approved raw material for regenerative medicine by Japan PMDA with a "再生医療等製品材料適格性確認書" certificate.

Effects of various albumins on hMSC culture

Order deAlbumin

SKU Availability Format Application Price(USD) Quantity
DA01-1G > 10 Lyophilized Neuron, MSC, CM 90.0
DA01-20-25ML > 10 20% in DPBS Neuron, MSC, CM 380.0
DA07-20-25ML > 10 20% in DPBS T-cell, MSC 380.0

Notices to Buyers

   Items are shipped from Taiwan, every Monday and Tuesday by FedEx Intl. Priority. The shipping cost and tariff are included in our invoice.

Occasionally, FedEx would request the end-user to declare the intended use to the U.S. Customs and Border Protection (CBP).

Properties

Biological source
human, recombinant expressed in Pichia pastoris
Assay
≥98% Albumin, ≥90% monomer by PAGE
Mol. wt
monomer ~67 kDa
Endotoxin
≤1 EU/mg protein
Storage temp.
2-8°C
Gene
ALB (Human)

Documentation

References

  1. Estève, D. et al. Stem Cells International 2016, 1–8 (2016). Pubmed
  2. Schnitzler, A. C. et al. Biochemical Engineering Journal 108, 3–13 (2016). Link
  3. Fillmore, N. et al. PLOS ONE 10, e0120257 (2015). Pubmed